To more accurately show how grueling it was for his character to survive, Vincent Price insisted on lifting real people into the back of his car instead of dummies. This is why it seems he's taking extra care with the bodies. For the scene at the pit, however, he's handling dummies for obvious reasons.

Established by many reviewers (including director George A. Romero himself) as a graphic blueprint for Night of the Living Dead (1968).

Although this is much more faithful to the book than The Omega Man (1971) and I Am Legend (2007) which are based on the same novel, it changes the main character's name from Robert Neville to Robert Morgan, while the other two leave it unchanged.

It is assumed that the action takes place in USA, but Italian cars (such as the Fiat 600 that were unusual in the States) appear, because the film was shot in Italy. Morgan drives a 1957 Chevrolet Station Wagon with California license plates, and the map he maintains is that of San Francisco CA, turned sideways.

The script was written in part by Richard Matheson, but he was dissatisfied with the result and was therefore credited as Logan Swanson.

The strange, tall, UFO or mushroomed shaped building in the background behind Dr. Robert Morgan is the "Ristorante IL Fungo" in Rome, built in 1957. It was restored in 2013-14.

Richard Matheson originally wrote the script in 1957, at which point it was to have been produced by Hammer films with Fritz Lang slated to direct.

Richard Matheson felt Vincent Price was miscast as Robert Morgan.

This film was originally going to be produced by Hammer Films of Great Britain. They decided not to make it and passed the script over to their U.S. associate, Robert L. Lippert, who produced the film in Italy.

The film takes place in 1968.

Hammer had tried to set this up in the late 50's as "Night Creatures." Stanley Baker, Paul Massie, Laurence Harvey and Kieron Moore were all considered for the Robert Neville part.

KOKW was the amateur (HAM) radio call sign used when Price was transmitting on the radio. The call sign does exist but is not listed to "The Last Man on Earth".

Charlton Heston viewed this film before proceeding with his remake The Omega Man (1971). He described this version as "incredibly botched, totally unfrightening, ill-acted, sloppily written and photographed."

During the scenes where Morgan is attacked by vampires, a score is played which is nearly identical to the music which plays during the Cormoran (giant) attack in 1962's Jack the Giant Killer.